The use of preservatives in food production, pharmaceutical and cosmetic industry is widespread. In cosmetic products they prevent spoilage, but also the growth of different types of microorganisms. The most famous preservative in the cosmetic industry is paraben, but because of its harmfulness to human health it was discarded from use and replaced by other harmless preservatives. Apart from synthetic replacements, natural preservatives are increasingly used, such as Geogard 221 which, as the active ingredient, contain dehydroxyacetic acid. The aim of this work was to explore the application of Geogarda 221 in the production of creams and prevent the growth of Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and Aspergillus brasiliensis. Samples of different types of creams contained 2mg/g of Geogarda 221 and were inoculated with test microorganisms in an amount of 5 to 7 logCFU/g. Contaminated samples of creams were kept at room temperature and analyzed 0, 2,7, 14 and 28 days. After the 2nd day of guarding number of microorganisms dropped by about 2 to 3 (DlogCFU/g). After the 7th day of guarding in samples of the cream no test microorganisms were found. The results obtained show effective action of Geogarda 221 on different microorganisms and when they are present in high concentrations.