A one-year study of the Fire Salamander (Salamandra salamandra) was conducted by visual observation on foot during 2019. and 2020. on the part of the road for panoramic trains (2,400 m) in the Plitvice Lakes National Park with the aim of defining weather conditions for daily and seasonal activities, identification of migration routes, defining the spatial distribution of roadkills (critical points) and proposing conservation and mitigation measures for reduceing roadkills. Out of a total of 126 recorded individuals of 5 amphibian species, 72 individuals were roadkilled. Fire Salamander is a species that is represented in total with 86 % of which 43 % are alive and roadkilled, while the remaining 14 % of victims belong to the species: common toad, yellow-bellied toad, smooth newt and common frog. According to the share of species of roadkilled individuals, the fire salamander is the most represented with 75 %. The highest number of roadkilled fire salamanders was recorded during the autumn migration in September, and alive during the spring migration in April. During the spring of 2020., due to the COVID-19 pandemic, traffic in the Park was closed, which possibly affected the results of the research. Most roadkilled and living individuals were found at temperatures of 8 - 14 ° C and relative humidity higher than 60 %. Most live individuals are active between 9 AM and 10 AM. Most of the roadkild and living individuals were found in the direction of movement land-lake during spring migration. Females are of bigger average overall length, length from the tip of the muzzle to the end of the cloaca and weight than males. Picture documentation (dorsal and ventral sides) did not identify two of the same individuals. Using ArcMap 10.5.1. program, the spatial distribution of roadkilled and living data was grouped and eight "hot spots" were assumed. Measures of protection and reduceing roadkills are proposed: placing signs to warn drivers of the presence of animals, setting up temporary fences with a buckets, limiting vehicle speed, closing or restricting traffic and combination of permanent and temporary fences with buckets/funnel traps for amphibiants.